Photostimulation of osteogenic differentiation on silk scaffolds by plasma arc light source

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dc.contributor.authorÇakmak A.S.
dc.contributor.authorÇakmak S.
dc.contributor.authorVatansever H.S.
dc.contributor.authorGümüşderelioğlu M.
dc.date.accessioned2024-07-22T08:09:57Z
dc.date.available2024-07-22T08:09:57Z
dc.date.issued2018
dc.description.abstractLow-level laser therapy (LLLT) has been used for more than 30 years to heal wounds. In recent years, LLLT or photostimulation has been indicated as an effective tool for regenerative and dental medicine by using monochromatic light. The aim of this study is to indicate the usability of plasma arc light source for bone regeneration. This is why we used polychromatic light source providing effective wavelengths in the range of 590–1500 nm for cellular response and investigated photostimulation effects on osteogenic differentiation of human mesenchymal stem cells (hMSCs) seeded on 3D silk scaffolds. Cellular responses were examined by using cell culture methods in terms of proliferation, differentiation, and morphological analyses. The results showed that photostimulation with a polychromatic light source (applied for 5 min from the 3rd day after seeding up to the 28th day in 2-day intervals with 92-mW/cm2 power from 10-cm distance to the cells) enhanced osteogenic differentiation of hMSCs according to higher alkaline phosphatase (ALP) activity, collagen and calcium content, osteogenic gene expressions, and matrix mineralization. In conclusion, we suggest that the plasma arc light source that was used here has a great potential for bone regeneration. © 2017, Springer-Verlag London Ltd., part of Springer Nature.
dc.identifier.DOI-ID10.1007/s10103-017-2414-4
dc.identifier.issn02688921
dc.identifier.urihttp://akademikarsiv.cbu.edu.tr:4000/handle/123456789/15019
dc.language.isoEnglish
dc.publisherSpringer London
dc.subjectAlkaline Phosphatase
dc.subjectBone Regeneration
dc.subjectCalcium
dc.subjectCell Culture Techniques
dc.subjectCell Differentiation
dc.subjectCell Proliferation
dc.subjectCells, Cultured
dc.subjectCollagen
dc.subjectHumans
dc.subjectInfrared Rays
dc.subjectLow-Level Light Therapy
dc.subjectMesenchymal Stromal Cells
dc.subjectOsteogenesis
dc.subjectSilk
dc.subjectTissue Scaffolds
dc.subjectalkaline phosphatase
dc.subjectcalcium
dc.subjectcollagen
dc.subjectDNA
dc.subjectsilk fibroin
dc.subjectalkaline phosphatase
dc.subjectcalcium
dc.subjectcollagen
dc.subjectsilk
dc.subjectArticle
dc.subjectbone development
dc.subjectbone mineralization
dc.subjectbone regeneration
dc.subjectcell differentiation
dc.subjectcell proliferation
dc.subjectcell structure
dc.subjectcell viability
dc.subjectconfocal microscopy
dc.subjectcontrolled study
dc.subjectenergy dispersive X ray spectroscopy
dc.subjectenzyme activity
dc.subjectgene expression
dc.subjecthuman
dc.subjecthuman cell
dc.subjectlight
dc.subjectmesenchymal stem cell
dc.subjectplasma arc light source
dc.subjectpriority journal
dc.subjectreverse transcription polymerase chain reaction
dc.subjectscanning electron microscopy
dc.subjectstem cell culture
dc.subjectbone development
dc.subjectcell culture
dc.subjectcell culture technique
dc.subjectcell differentiation
dc.subjectcytology
dc.subjectinfrared radiation
dc.subjectlow level laser therapy
dc.subjectmesenchymal stroma cell
dc.subjectmetabolism
dc.subjectphysiology
dc.subjectradiation response
dc.subjecttissue scaffold
dc.subjectultrastructure
dc.titlePhotostimulation of osteogenic differentiation on silk scaffolds by plasma arc light source
dc.typeArticle

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