A comparative study of antimicrobial activity using well and disk diffusion method on Asphodelus aestivus (Liliaceae)
No Thumbnail Available
Date
Journal Title
Journal ISSN
Volume Title
Abstract
Plant parts were powdered, extracted in a Soxhlet extractor with n-butanol and ethanol (60-80 degrees C) for 12 h, filtered using Whatman filter paper no:4 and the filtrate was then evaporated at 70 degrees C. For determination of antimicrobial activity, different concentrations of crude extract tested against bacteria and yeasts by both well and disk diffusion method. Results showed that the A phodelus aestivus extract (Flower, fruits and whole plant) exhibited antimicrobial activity ranging from 2 to 15 mm. Extract obtained with ethanol was showed more inhibision. According to same concentrations of crude extract used with well diffusion method results were a bit higher than disc diffusion results. Minimal inhibitory concentration (MIC) determined on susceptible microorganisms by macrobroth dilution method, and MIC values were shown from 36 to 90 mg/mL. Also some commercial antibiotics were used to determine sensitivity of the test microorganisms and comparison.