Analysis of the Effects of Inhibitor and Activator Systems (Smad's Proteins) of TGF-βs on Chick Neural Tube Closure
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Abstract
The families of TGF-beta s and Smads proteins that controls its intracellular signaling pathways are known to play a role in early neurulation. The aim of this study is to demonstrate distribution of TGF-beta s (1, 2, 3) and Smads (1/2/3, 6, 7) proteins as a system in different hours of neural tube development of chick embryos. The SPF eggs were incubated at 37.8 +/- 2 degrees C for 24(th), 30(th), 48(th), 72(nd) h. After that, embryos were examined using immunohistochemistry and western blotting techniques. To the results, TGF-beta s immunoreactivities (particularly TGF-beta 3) at the 24(th), 30(th) and 48(th) h of chick development (during neural tube closure) were determined and decreased at the 72nd h (after neural tube closure), but expressions of TGF-beta s were detected in all stage of embryos in western blotting. While Smad 1/2/3 immunoreactivitiy and expression was less than that of the Smad 6 and 7 at the 24(th), it was increased at the 30(th) h. Smads proteins immunoreactivities were decresead at the 72(nd) h. In conclusion, the members of TGF-beta s are play a role in chick neural tube closure, the secretions of TGF-beta s are controlled different Smad proteins. In addition, immunoblotting results showed that TGF-beta s and Smads proteins were effective in the development of all tissues and organs of the embryos.
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GROWTH-FACTOR-BETA , NERVOUS-SYSTEM , LOCALIZATION , EXPRESSION , ROLES , TGF-BETA-1 , PATTERNS , EMBRYO