Comparative phytochemical studies on the roots of Polygala azizsancarii and P. peshmenii and neuroprotective activities of the two xanthones

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dc.contributor.authorÇalis, I
dc.contributor.authorBecer, E
dc.contributor.authorÜnlü, A
dc.contributor.authorAydin, ZU
dc.contributor.authorHanoglu, A
dc.contributor.authorVatansever, HS
dc.contributor.authorDönmez, AA
dc.date.accessioned2024-07-18T12:05:35Z
dc.date.available2024-07-18T12:05:35Z
dc.description.abstractSix known sucrose mono-, di- and triesters and five xanthone derivatives were isolated from the roots of Polygala peshmenii Eren, Parolly, Raus & Kurschner which is a narrow species endemic to Turkiye. Among the xanthones, 1,7-dihydroxy-2,3-methylenedioxy-5,6-dimethoxy-xanthone is an undescribed compound isolated for the first time from a natural source. The studies on the roots of P. azizsancarii Do & BULL;nmez have resulted in the isolation of four known compounds including sucrose mono-, di- and triesters. The structures of the sucrose esters and xanthones isolated from P. azizsancarii and P. peshmenii were established by spectroscopic methods, including 1DNMR (1H NMR, 13C NMR, DEPT-135), 2D-NMR (COSY, NOESY, HSQC, HMBC). Neuroprotective activities of two xanthones, 1,3,6-trihydroxy-2,5,7-trimethoxyxanthone and 3-O-& beta;-D-glucopyranosyloxy-1,6-dihydroxy-2,5,7-trimethoxyxanthone isolated from the roots of P. azizsancarii were evaluated in vitro using in a cellular model of Alzheimer's disease. SKNAS human neuroblastoma cells were used in the study and treated with different consecrations of A & beta;25-35 oligomer for up to 48 h. Cell viability was evaluated using MTT assay. The distribution of & beta;-amyloid, & alpha;-synuclein, tau, JAK2, STAT3, caspase 3 and BMP-2 were investigated using indirect immunoperoxidase staining. Our results suggested that both xanthones control tau aggregation with no effect on & beta;-amyloid plaque formation. In addition, for neuronal pathophysiology in AD cell model, decreased distributions of JAK/ STAT3 and BMP2 signaling pathways were demonstrated, therefore they play a role in the protective effect on neurons in neurodegenerative disease. A significant decrease in caspase 3 immunoreactivity was detected after the administration of both compounds in AD cells. Therefore, both compounds control neuronal pathophysiology and rescue cell death in AD disease.
dc.identifier.issn0031-9422
dc.identifier.other1873-3700
dc.identifier.urihttp://akademikarsiv.cbu.edu.tr:4000/handle/123456789/9868
dc.language.isoEnglish
dc.publisherPERGAMON-ELSEVIER SCIENCE LTD
dc.subjectOLIGOSACCHARIDE MULTI-ESTERS
dc.subjectPERFORMANCE LIQUID-CHROMATOGRAPHY
dc.subjectVAR LATIFOLIA TORR
dc.subjectALZHEIMERS-DISEASE
dc.subjectTRITERPENOID SAPONINS
dc.subjectTENUIFOLIA WILLDENOW
dc.subjectPHENOLIC GLYCOSIDES
dc.subjectBIOACTIVE SAPONINS
dc.subjectAERIAL PARTS
dc.subjectTAU-PROTEIN
dc.titleComparative phytochemical studies on the roots of Polygala azizsancarii and P. peshmenii and neuroprotective activities of the two xanthones
dc.typeArticle

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