Effects of 5-fluorouracil and gemcitabine on a breast cancer cell line (MCF-7) via the JAK/STAT pathway

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dc.contributor.authorUluer, ET
dc.contributor.authorAydemir, I
dc.contributor.authorInan, S
dc.contributor.authorOzbilgin, K
dc.contributor.authorVatansever, HS
dc.date.accessioned2024-07-18T11:54:01Z
dc.date.available2024-07-18T11:54:01Z
dc.description.abstractAberrant activation of the JAK/STAT pathway may predispose to malignancy as a consequence of the deregulation of cell proliferation, differentiation or apoptosis such as in cancer of the blood, head and neck, and breast. In our study we aimed to investigate the effects of 5-fluorouracil (5-FU) and gemcitabine on a breast cancer cell line (MCF-7 cells) via the JAK/STAT pathway. Distribution of JAK1, JAK2, JAK3 and STAT2, STAT3, STAT4, STAT5 were evaluated on MCF-7 cells following gemcitabine and 5-FU treatment and in the absence of drug treatment by an indirect immunohistochemical method. It was observed that JAK1, JAK3, STAT5 and particularly STAT2 activation were more effective than the other JAK/STATs in breast cancer progression. Following treatment with 5-FU, JAK1 and STAT5 immunoreactivities were decreased in MCF-7 cells in comparison with both gemcitabine-treated and non-treated groups. These results suggest that the JAK/STAT pathway plays an important role in breast cancer pathogenesis and may be more affected after 5-FU treatment rather than gemcitabine. Drugs which block STAT5 may provide a novel therapeutic approach for the treatment of breast cancer. (c) 2011 Elsevier GmbH. All rights reserved.
dc.identifier.issn0065-1281
dc.identifier.other1618-0372
dc.identifier.urihttp://akademikarsiv.cbu.edu.tr:4000/handle/123456789/6054
dc.language.isoEnglish
dc.publisherELSEVIER GMBH
dc.subjectMAMMARY-GLAND DEVELOPMENT
dc.subjectSIGNAL-TRANSDUCER
dc.subjectTRANSCRIPTIONAL CONTROL
dc.subjectSTAT PROTEINS
dc.subjectFAMILY
dc.subjectROLES
dc.subjectACTIVATION
dc.subjectEXPRESSION
dc.subjectDIFFERENTIATION
dc.subjectSURVIVAL
dc.titleEffects of 5-fluorouracil and gemcitabine on a breast cancer cell line (MCF-7) via the JAK/STAT pathway
dc.typeArticle

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