Histological and electroencephalographic demonstration of probiotic effect for reduce of oxidative stress and apoptosis in experimental traumatic brain injury

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dc.contributor.authorKarakayali, EM
dc.contributor.authorKocamaz, E
dc.contributor.authorAlpay, S
dc.contributor.authorOnal, T
dc.contributor.authorOztatlici, M
dc.contributor.authorDurusma, R
dc.contributor.authorOzel, HF
dc.contributor.authorMete, M
dc.contributor.authorBarutcuoglu, M
dc.contributor.authorKutlu, N
dc.contributor.authorTuglu, MI
dc.date.accessioned2024-07-18T12:05:21Z
dc.date.available2024-07-18T12:05:21Z
dc.description.abstractBACKGROUND: The gut microbiota modulates nervous system function. In the literature, it has been shown that this modula-tion is used in many nervous system injuries through oxidative stress (OS) and apoptosis mechanisms. In this study, it was aimed to investigate the neuroprotective effects of probiotic (PB) treatment in a rat traumatic brain injury (TBI) model with histological and electroencephalographic (EEG) data.METHODS: Forty male Wistar albino rats were divided into four groups. Group 1 was the control group (CONTROL, n=10) and no trauma was applied. Group 2 was the trauma group with the weight-drop technique (TBH, n=10). Group 3 was the sham group (SHAM), (TBH+sterile saline [SS], n=10) rats were given 500 mu L of SS per day by oral gavage. Group 4 was the PB treatment group, (TBH+PB, n=10) rats were treated daily for 7 days with 500 mu L of PB oral gavage. Brain samples were collected 7 days after trauma. Histopathological evaluation of brain samples was done with HE. OS with Endothelial nitric oxide synthase, vascularization with Vascular Endothelial Growth Factor, gliosis with S100, and apoptosis with caspase 3 were evaluated immunohistochemically. Apoptotic index was determined with TUNEL. In addition, EEG and somatosensory evoked potential (SEP) recording findings were compared.RESULTS: It was determined by HE staining that there was a significant (P<0.001) damage in the TBI and sham groups compared to the control group. It was found that PB treatment provided a significant (P<0.01) improvement in the damage created. While OS (P<0.01), gliosis (P<0.01), and apoptosis (P<0.05) decreased with PB treatment, angiogenesis (P<0.01) increased. In support of these findings, in the software-mediated EEG and SUP examination; Delta wave power and theta/alpha ratio increased with TBI and de-creased with PB treatment.CONCLUSION: The results showed that PB treatment provided a significant improvement in rats by reducing OS, apoptosis, and gliosis and increasing vascularity. To the best of our knowledge in the literature, it was shown for the 1st time that histological results for the treatment of PB were supported by software-mediated EEG and SEP analysis.
dc.identifier.issn1306-696X
dc.identifier.other1307-7945
dc.identifier.urihttp://akademikarsiv.cbu.edu.tr:4000/handle/123456789/9691
dc.language.isoEnglish
dc.publisherTURKISH ASSOC TRAUMA EMERGENCY SURGERY
dc.subjectGUT MICROBIOTA
dc.titleHistological and electroencephalographic demonstration of probiotic effect for reduce of oxidative stress and apoptosis in experimental traumatic brain injury
dc.typeArticle

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