The effects of DBcAMP on the expression of eNOS, iNOS and TGF-beta in rat heart tissue

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dc.contributor.authorEkerbicer, N
dc.contributor.authorInan, S
dc.contributor.authorTarakci, F
dc.contributor.authorZeren, T
dc.date.accessioned2024-07-18T11:47:21Z
dc.date.available2024-07-18T11:47:21Z
dc.description.abstractDibutryl (DB) adenosine 3',5'-cyclic monophosphate (cAMP) is an important modulator of physiological functions. To determine the protective effects of DBcAMP on heart tissue, we evaluated changes in immunoreactivity of endothelial nitric oxide synthase (eNOS), inducible nitric oxide synthase (iNOS) and transforming growth factor-beta (TGF-) in left cervical vagotomized rats treated with DBcAMP. Male rats were divided into four groups. In Group 1, animals were subjected to a left cervical vagotomy. Group 2 received a 1 ml bolus injection of 15 ml/kg DBcAMP in addition to the left vagotomy. DBcAMP alone was given to Group 3 and Group 4 was the control group. For each animal, mean arterial pressure (MAP) and heart rate (HR) were measured. For indirect immunohistochemistry, anti-eNOS, anti-iNOS, and anti-TGF- primary antibodies were used. In Group 1, MAP and HR values decreased slightly. In Groups 2 and 3, DBcAMP induced a statistically significant drop in HR and MAP. In Group 1, strong eNOS, iNOS, and TGF- immunoreactivities were observed. Immunostaining intensities decreased in Groups 2 and 3. The results of the study reported here suggest that increased immunoreactivities of eNOS, iNOS, and TGF- might contribute to the effects on the heart tissue after left vagotomy and imply that DBcAMP acts on heart tissue via nitric oxide.
dc.identifier.issn1052-0295
dc.identifier.other1473-7760
dc.identifier.urihttp://akademikarsiv.cbu.edu.tr:4000/handle/123456789/3464
dc.language.isoEnglish
dc.publisherTAYLOR & FRANCIS LTD
dc.subjectNITRIC-OXIDE SYNTHASE
dc.subjectDIBUTYRYL-CYCLIC-AMP
dc.subjectCARDIAC MYOCYTES
dc.subjectCAMP
dc.subjectINHIBITION
dc.subjectFAILURE
dc.subjectPATHWAY
dc.subjectCELLS
dc.subjectDOG
dc.titleThe effects of DBcAMP on the expression of eNOS, iNOS and TGF-beta in rat heart tissue
dc.typeArticle

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