Preparation, in vitro mineralization and osteoblast cell response of electrospun 13-93 bioactive glass nanofibers
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2015
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Abstract
In this study, silicate based 13-93 bioactive glass fibers were prepared through sol-gel processing and electrospinning technique. A precursor solution containing poly (vinyl alcohol) and bioactive glass sol was used to produce fibers. The mixture was electrospun at a voltage of 20 kV by maintaining tip to a collector distance of 10 cm. The amorphous glass fibers with an average diameter of 464 ± 95 nm were successfully obtained after calcination at 625 °C. Hydroxyapatite formation on calcined 13-93 fibers was investigated in simulated body fluid (SBF) using two different fiber concentrations (0.5 and 1 mg/ml) at 37 °C. When immersed in SBF, conversion to a calcium phosphate material showed a strong dependence on the fiber concentration. At 1 mg/ml, the surface of the fibers converted to the hydroxyapatite-like material in SBF only after 30 days. At lower solid concentrations (0.5 mg/ml), an amorphous calcium phosphate layer formation was observed followed by the conversion to hydroxyapatite phase after 7 days of immersion. The XTT (2,3-Bis-(2-Methoxy-4-Nitro-5-Sulfophenyl)-2H-Tetrazolium-5-Carboxanilide) assay was conducted to evaluate the osteoblast cell response to the bioactive glass fibers. © 2015 Elsevier B.V. All rights reserved.
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Animals , Biocompatible Materials , Cell Line , Cell Survival , Electrochemical Techniques , Glass , Mice , Nanofibers , Osteoblasts , Particle Size , Surface Properties , Calcination , Calcium , Calcium phosphate , Cells , Electrospinning , Fibers , Glass fibers , Hydroxyapatite , Polyvinyl alcohols , Silicates , Sol-gel process , Sol-gels , Spinning (fibers) , Tissue engineering , 13-93 bioactive glass , biomaterial , glass , nanofiber , Amorphous calcium phosphate , Calcium phosphate materials , Electrospinning techniques , Hydroxyapatite formations , Precursor solutions , Simulated body fluids , Solid concentrations , Tissue engineering applications , animal , cell line , cell survival , chemistry , drug effects , electrochemical analysis , mouse , osteoblast , particle size , surface property , Bioactive glass