Never in mitosis gene A-related kinase 6 and aurora kinase A: New gene biomarkers in the conversion from ulcerative colitis to colorectal cancer
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Date
2015
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Abstract
Ulcerative colitis (UC) is an important risk factor for colorectal cancer (CRC). Histone modifications are one of the epigenetic mechanisms that may have key roles in the carcinogenesis of CRC. At present, there are no studies comparing histone modification patterns of UC and CRC in the literature. Therefore the aim of the present study was to investigate whether genes, particularly those involved in histone modification, have value in patient monitoring with regards to CRC development in UC. Key gene expressions of the histone modification enzyme were assessed and compared in CRC, UC and control groups using the RT-PCR array technique. Patients were divided into subgroups based on the extent and duration of the disease and inflammatory burden, which are considered risk factors for CRC development in UC patients. In UC and CRC groups, a significantly higher overexpression of the NEK6 and AURKA genes compared to the control group was identified. In addition, there was a significantly higher overexpression of HDAC1 and PAK1 genes in the UC group, and of HDAC1, HDAC7, PAK1 and AURKB genes in the CRC group. NEK6, AURKA, HDAC1 and PAK1 were significantly overexpressed in patients with a longer UC duration. Overexpression of AURKA and NEK6 genes was significantly more pronounced in UC patients with more extensive colon involvement. HDAC1, HDAC7, PAK1, NEK6, AURKA and AURKB are important diagnostic and prognostic markers involved in the carcinogenesis of CRC. HDAC1, PAK1, NEK6 and AURKA may be considered as diagnostic markers to be used in CRC screening for UC patients.
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Keywords
Adult , Aged , Aurora Kinase A , Biomarkers, Tumor , Cell Transformation, Neoplastic , Colitis, Ulcerative , Colorectal Neoplasms , DNA Methylation , Epigenesis, Genetic , Female , Histone Code , Histone Deacetylase 1 , Histone Deacetylases , Humans , Male , Middle Aged , p21-Activated Kinases , Protein-Serine-Threonine Kinases , aurora A kinase , biological marker , histone deacetylase 1 , histone deacetylase 7 , p21 activated kinase 1 , aurora A kinase , HDAC1 protein, human , HDAC7 protein, human , histone deacetylase , histone deacetylase 1 , NEK6 protein, human , p21 activated kinase , PAK1 protein, human , protein serine threonine kinase , tumor marker , adult , Article , AURKB gene , aurora kinase A gene , cancer growth , cancer prognosis , cancer risk , colon carcinogenesis , colorectal cancer , controlled study , disease duration , disease severity , enzyme analysis , female , gene overexpression , genetic analysis , HDAC1 gene , HDAC7 gene , histone modification , histopathology , human , human tissue , major clinical study , male , malignant transformation , never in mitosis gene A related kinase 6 gene , PAK1 gene , patient monitoring , priority journal , reverse transcription polymerase chain reaction , ulcerative colitis , aged , biosynthesis , cell transformation , Colorectal Neoplasms , complication , DNA methylation , genetic epigenesis , genetics , histone code , middle aged , pathology , ulcerative colitis