COMET, TUNEL, and TEM analysis of an infertile male with short tail sperm
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Date
2015
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Abstract
Male infertility is correlated with sperm morphology and sperm DNA damage, which are completely different from that of fertile individuals. An accurate sperm DNA damage analysis and ultrastructural examination of the ejaculate provide important support in the clinical evaluation. It is supposed that in the near future, the fertilization rate, pregnancy rate, and miscarriages could be predicted using the combination of these types of tests in assisted reproductive technologies (ARTs). For this purpose, we report a very rare case of an infertile man having short tail sperm. The infertile man and his wife underwent in vitro fertilization (IVF) with intracytoplasmic sperm injection (ICSI). During this process, we examined the ultrastructure of the ejaculated sperm with transmission electron microscopy (TEM) and calculated the sperm DNA damage with terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) and COMET assays. Then, we evaluated the association between sperm DNA damage and embryo quality. © 2015 by the Turkish-German Gynecological Education and Research Foundation.
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Keywords
eosin , adult , Article , asthenospermia , case report , comet assay , disease severity , DNA damage , DNA fragmentation , fertilization in vitro , human , intracytoplasmic sperm injection , karyotype 46,XX , karyotype 46,XY , male , male genital tract parameters , male infertility , masturbation , nick end labeling , rare disease , semen analysis , sexual abstinence , short tail sperm , smoking , spermatozoon abnormality , spermatozoon count , spermatozoon head , spermatozoon motility , transmission electron microscopy