COMET, TUNEL, and TEM analysis of an infertile male with short tail sperm

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dc.contributor.authorDurmaz A.
dc.contributor.authorMıçılı S.C.
dc.contributor.authorVatansever S.
dc.contributor.authorGündüz C.
dc.contributor.authorBağrıyanık H.A.
dc.contributor.authorDikmen N.
dc.contributor.authorTavmergen Göker E.N.
dc.contributor.authorTavmergen E.
dc.date.accessioned2024-07-22T08:14:37Z
dc.date.available2024-07-22T08:14:37Z
dc.date.issued2015
dc.description.abstractMale infertility is correlated with sperm morphology and sperm DNA damage, which are completely different from that of fertile individuals. An accurate sperm DNA damage analysis and ultrastructural examination of the ejaculate provide important support in the clinical evaluation. It is supposed that in the near future, the fertilization rate, pregnancy rate, and miscarriages could be predicted using the combination of these types of tests in assisted reproductive technologies (ARTs). For this purpose, we report a very rare case of an infertile man having short tail sperm. The infertile man and his wife underwent in vitro fertilization (IVF) with intracytoplasmic sperm injection (ICSI). During this process, we examined the ultrastructure of the ejaculated sperm with transmission electron microscopy (TEM) and calculated the sperm DNA damage with terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) and COMET assays. Then, we evaluated the association between sperm DNA damage and embryo quality. © 2015 by the Turkish-German Gynecological Education and Research Foundation.
dc.identifier.DOI-ID10.5152/jtgga.2014.0017
dc.identifier.issn13090399
dc.identifier.urihttp://akademikarsiv.cbu.edu.tr:4000/handle/123456789/16625
dc.language.isoEnglish
dc.publisherAVES Ibrahim Kara
dc.rightsAll Open Access; Gold Open Access
dc.subjecteosin
dc.subjectadult
dc.subjectArticle
dc.subjectasthenospermia
dc.subjectcase report
dc.subjectcomet assay
dc.subjectdisease severity
dc.subjectDNA damage
dc.subjectDNA fragmentation
dc.subjectfertilization in vitro
dc.subjecthuman
dc.subjectintracytoplasmic sperm injection
dc.subjectkaryotype 46,XX
dc.subjectkaryotype 46,XY
dc.subjectmale
dc.subjectmale genital tract parameters
dc.subjectmale infertility
dc.subjectmasturbation
dc.subjectnick end labeling
dc.subjectrare disease
dc.subjectsemen analysis
dc.subjectsexual abstinence
dc.subjectshort tail sperm
dc.subjectsmoking
dc.subjectspermatozoon abnormality
dc.subjectspermatozoon count
dc.subjectspermatozoon head
dc.subjectspermatozoon motility
dc.subjecttransmission electron microscopy
dc.titleCOMET, TUNEL, and TEM analysis of an infertile male with short tail sperm
dc.typeArticle

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