Fatty acid composition of Opuntia ficus-indica seed oil control angiogenic activity in colon carcinoma cell lines
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Date
2021
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Abstract
Opuntia ficus-indica belongs to the family Cactaceae that particularly rich in unsaturated fatty acids. The aim of this study was to investigate the composition and in vitro anti-angiogenic activity of spiny and thornless Opuntia ficus-indica seed (CPS) oils. Spiny and thornless CPS oils were obtained by supercritical CO2 extraction and analyzed by GC-MS. Cell growth and cytotoxicity were measured with MTT assay with different concentrations of spiny and thornless CPS oils in Colo-320 and Colo-741 cell lines. Anti-angiogenic activity of CPS oils were investigated by immunocytochemistry using antibodies directed against to Flt- 1, Flk-1, eNOS, iNOS, IL-6, PGE2, TNF-α and VEGF. Oleic acid (17.6%; 15.6%), linoleic acid (55.9%; 60.1%), palmitic acid (12.4%; 12.3%), elaidic acid (4%; 4.1%) were determined as the major compounds of spiny and thornless CPS oils, respectively. In the MTT assay, 1:16 dilution spiny CPS oil was found to be active against Colo-320 and Colo-741 cells for 48h incubation. Also, 1:8 and 1:16 dilutions of thornless CPS oil were more effective at inhibiting Colo-320 and Colo-741 for 48 h, respectively.We conclude that spiny CPS oil decreased signaling molecules which play in PGE2 mediated and VEGF-dependent angiogenesis. Interestingly, thornless CPS oil increased angiogenesis thought signaling molecules in colon cancer cells. © 2021 Mattioli 1885. All rights reserved.
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elaidic acid , endothelial nitric oxide synthase , fatty acid , inducible nitric oxide synthase , interleukin 6 , Ki 67 antigen , linoleic acid , oleic acid , palmitic acid , plant extract , prostaglandin E2 , tumor necrosis factor , vasculotropin , vasculotropin receptor 1 , vasculotropin receptor 2 , vegetable oil , antiangiogenic activity , antigen expression , antineoplastic activity , antiproliferative activity , Article , cancer inhibition , cell growth , cell proliferation , COLO 320 cell line , colon carcinoma , colon carcinoma cell line , concentration (parameter) , controlled study , dilution , drug cytotoxicity , drug determination , drug identification , drug isolation , drug mechanism , human , human cell , immunocytochemistry , in vitro study , lipid composition , mass fragmentography , migration inhibition , MTT assay , Opuntia ficus-indica