Fatty acid composition of Opuntia ficus-indica seed oil control angiogenic activity in colon carcinoma cell lines

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dc.contributor.authorBecer E.
dc.contributor.authorKabadayi H.
dc.contributor.authorMeriçli A.H.
dc.contributor.authorKivançli B.
dc.contributor.authorVatansever H.S.
dc.contributor.authorMeriçli F.
dc.date.accessioned2024-07-22T08:06:13Z
dc.date.available2024-07-22T08:06:13Z
dc.date.issued2021
dc.description.abstractOpuntia ficus-indica belongs to the family Cactaceae that particularly rich in unsaturated fatty acids. The aim of this study was to investigate the composition and in vitro anti-angiogenic activity of spiny and thornless Opuntia ficus-indica seed (CPS) oils. Spiny and thornless CPS oils were obtained by supercritical CO2 extraction and analyzed by GC-MS. Cell growth and cytotoxicity were measured with MTT assay with different concentrations of spiny and thornless CPS oils in Colo-320 and Colo-741 cell lines. Anti-angiogenic activity of CPS oils were investigated by immunocytochemistry using antibodies directed against to Flt- 1, Flk-1, eNOS, iNOS, IL-6, PGE2, TNF-α and VEGF. Oleic acid (17.6%; 15.6%), linoleic acid (55.9%; 60.1%), palmitic acid (12.4%; 12.3%), elaidic acid (4%; 4.1%) were determined as the major compounds of spiny and thornless CPS oils, respectively. In the MTT assay, 1:16 dilution spiny CPS oil was found to be active against Colo-320 and Colo-741 cells for 48h incubation. Also, 1:8 and 1:16 dilutions of thornless CPS oil were more effective at inhibiting Colo-320 and Colo-741 for 48 h, respectively.We conclude that spiny CPS oil decreased signaling molecules which play in PGE2 mediated and VEGF-dependent angiogenesis. Interestingly, thornless CPS oil increased angiogenesis thought signaling molecules in colon cancer cells. © 2021 Mattioli 1885. All rights reserved.
dc.identifier.DOI-ID10.23751/pn.v23i2.10042
dc.identifier.issn11298723
dc.identifier.urihttp://akademikarsiv.cbu.edu.tr:4000/handle/123456789/13434
dc.language.isoEnglish
dc.publisherMattioli 1885
dc.subjectelaidic acid
dc.subjectendothelial nitric oxide synthase
dc.subjectfatty acid
dc.subjectinducible nitric oxide synthase
dc.subjectinterleukin 6
dc.subjectKi 67 antigen
dc.subjectlinoleic acid
dc.subjectoleic acid
dc.subjectpalmitic acid
dc.subjectplant extract
dc.subjectprostaglandin E2
dc.subjecttumor necrosis factor
dc.subjectvasculotropin
dc.subjectvasculotropin receptor 1
dc.subjectvasculotropin receptor 2
dc.subjectvegetable oil
dc.subjectantiangiogenic activity
dc.subjectantigen expression
dc.subjectantineoplastic activity
dc.subjectantiproliferative activity
dc.subjectArticle
dc.subjectcancer inhibition
dc.subjectcell growth
dc.subjectcell proliferation
dc.subjectCOLO 320 cell line
dc.subjectcolon carcinoma
dc.subjectcolon carcinoma cell line
dc.subjectconcentration (parameter)
dc.subjectcontrolled study
dc.subjectdilution
dc.subjectdrug cytotoxicity
dc.subjectdrug determination
dc.subjectdrug identification
dc.subjectdrug isolation
dc.subjectdrug mechanism
dc.subjecthuman
dc.subjecthuman cell
dc.subjectimmunocytochemistry
dc.subjectin vitro study
dc.subjectlipid composition
dc.subjectmass fragmentography
dc.subjectmigration inhibition
dc.subjectMTT assay
dc.subjectOpuntia ficus-indica
dc.titleFatty acid composition of Opuntia ficus-indica seed oil control angiogenic activity in colon carcinoma cell lines
dc.typeArticle

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