Immunohistochemical determination of mTOR pathway molecules in ovaries and uterus in rat estrous cycle stages

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dc.contributor.authorEkizceli, G
dc.contributor.authorInan, S
dc.contributor.authorOktem, G
dc.contributor.authorOnur, E
dc.contributor.authorOzbilgin, K
dc.date.accessioned2024-07-18T12:03:54Z
dc.date.available2024-07-18T12:03:54Z
dc.description.abstractmTOR is a member of the PI3K/Akt/mTOR signaling pathway that participates in cell growth, proliferation, protein synthesis, transcription, angiogenesis, apoptosis and autophagy. mTOR and MAPK pahways are two important key signal pathways which are related to each other. We investigated the role of mTOR and other signaling molecules in rat ovaries and uteruses in stages of the estrous cycle. Young adult female rats were divided into four groups as proestrous, estrous, metestrous and diestrous according to vaginal smears. Immunohistochemical staining of mTORC1, IGF1, PI3K, pAKT1/2/3, ERK1 and pERK1/2 was performed and pAKT1/2/3 and ERK1 were also analyzed using western blotting on ovarian and uterine tissue samples. According to our results, PI3K/Akt/ mTOR and ERK/pERK showed an increase in the rat ovulation period. When all the groups were evaluated the immunoreactivities for all of the antibodies were detected in the oocytes, granulosa and theca cells, corpus luteum and stroma of ovary and lamina propria, surface and glandular epithelium of uterus with the strongest observed with anti-ERK1 antibody and then with a decreasing trend with anti-mTORC1, anti-pAkt1/2/3, anti-IGF1, anti-PI3K and anti-pERK1/2 antibodies in the proestrus and estrus stages. Differently from other parts of the ovary, highest antibody expression in the corpus luteum was observed in the metestrous stage. Moreover, the existence of pAKT1/2/3 and ERK1 proteins was confirmed with the Western blotting technique. We suggest that mTOR and mTOR-related ERK signaling molecules may participate in the rat ovulation process.
dc.identifier.issn0213-3911
dc.identifier.other1699-5848
dc.identifier.urihttp://akademikarsiv.cbu.edu.tr:4000/handle/123456789/9441
dc.language.isoEnglish
dc.publisherF HERNANDEZ
dc.subjectINCREASED ACTIVATION
dc.subjectFOLLICLE ACTIVATION
dc.subjectMAMMALIAN TARGET
dc.subjectPI3K/AKT PATHWAY
dc.subjectGENE-EXPRESSION
dc.subjectGRANULOSA-CELLS
dc.subjectGROWTH-FACTORS
dc.subjectPTEN
dc.subjectTOR
dc.subjectFOLLICULOGENESIS
dc.titleImmunohistochemical determination of mTOR pathway molecules in ovaries and uterus in rat estrous cycle stages
dc.typeArticle

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