Liquidambar orientalis Mill. gum extract induces autophagy via PI3K/Akt/mTOR signaling pathway in prostate cancer cells
No Thumbnail Available
Date
2022
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
Abstract
Liquidambar orientalis Mill (LOM), is an endemic species having a local distribution in the southwestern coastal district of Turkey. Styrax liquidus gum (SLG), is a gum-like resinous which exudates in response to injury of the trunk of LOM. The aim of the study was to investigate the cytotoxic effects and the molecular mechanisms of the ethanolic SLG extract in human prostate cancer cells. GC-MS analysis was performed to identify the volatile compound composition. Cytotoxicity was determined by XTT analysis. Apoptosis and necrosis were evaluated via ELISA assay. Autophagic cell death was detected via monodansylcadaverine (MDC) staining and by measuring the levels of LC3I and LC3II. The protein levels of p-PI3K, p-Akt and p-mTOR were evaluated by western blot analysis. In the present study, it is shown that the SLG extract containing a considerable amount of ravidomycin derivate induced autophagic cell death in prostate cancer cells via inhibiting the PI3K/Akt/mTOR pathway. © 2020 Informa UK Limited, trading as Taylor & Francis Group.
Description
Keywords
Apoptosis , Autophagy , Humans , Liquidambar , Male , Phosphatidylinositol 3-Kinases , Plant Extracts , Prostatic Neoplasms , Proto-Oncogene Proteins c-akt , Signal Transduction , TOR Serine-Threonine Kinases , Turkey , alcohol , cytotoxic agent , dansylcadaverine , lc3i protein , lc3ii protein , Liquidambar orientalis extract , mammalian target of rapamycin , phosphatidylinositol 3 kinase , plant extract , protein , protein kinase B , unclassified drug , volatile agent , MTOR protein, human , phosphatidylinositol 3 kinase , plant extract , protein kinase B , target of rapamycin kinase , cancer , cell , endemic species , toxicity , Akt signaling , apoptosis , Article , autophagic cell death , cell viability , controlled study , cytotoxicity , DU145 cell line , embryo , enzyme linked immunosorbent assay , human , human cell , IC50 , Liquidambar , Liquidambar orientalis , male , mass fragmentography , PC-3 [Human prostate carcinoma] cell line , prostate cancer , tumor necrosis , Western blotting , XTT assay , autophagy , metabolism , prostate tumor , signal transduction