Liquidambar orientalis Mill. gum extract induces autophagy via PI3K/Akt/mTOR signaling pathway in prostate cancer cells

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dc.contributor.authorAtmaca H.
dc.contributor.authorCamli Pulat C.
dc.contributor.authorCittan M.
dc.date.accessioned2024-07-22T08:05:20Z
dc.date.available2024-07-22T08:05:20Z
dc.date.issued2022
dc.description.abstractLiquidambar orientalis Mill (LOM), is an endemic species having a local distribution in the southwestern coastal district of Turkey. Styrax liquidus gum (SLG), is a gum-like resinous which exudates in response to injury of the trunk of LOM. The aim of the study was to investigate the cytotoxic effects and the molecular mechanisms of the ethanolic SLG extract in human prostate cancer cells. GC-MS analysis was performed to identify the volatile compound composition. Cytotoxicity was determined by XTT analysis. Apoptosis and necrosis were evaluated via ELISA assay. Autophagic cell death was detected via monodansylcadaverine (MDC) staining and by measuring the levels of LC3I and LC3II. The protein levels of p-PI3K, p-Akt and p-mTOR were evaluated by western blot analysis. In the present study, it is shown that the SLG extract containing a considerable amount of ravidomycin derivate induced autophagic cell death in prostate cancer cells via inhibiting the PI3K/Akt/mTOR pathway. © 2020 Informa UK Limited, trading as Taylor & Francis Group.
dc.identifier.DOI-ID10.1080/09603123.2020.1818187
dc.identifier.issn09603123
dc.identifier.urihttp://akademikarsiv.cbu.edu.tr:4000/handle/123456789/13079
dc.language.isoEnglish
dc.publisherTaylor and Francis Ltd.
dc.subjectApoptosis
dc.subjectAutophagy
dc.subjectHumans
dc.subjectLiquidambar
dc.subjectMale
dc.subjectPhosphatidylinositol 3-Kinases
dc.subjectPlant Extracts
dc.subjectProstatic Neoplasms
dc.subjectProto-Oncogene Proteins c-akt
dc.subjectSignal Transduction
dc.subjectTOR Serine-Threonine Kinases
dc.subjectTurkey
dc.subjectalcohol
dc.subjectcytotoxic agent
dc.subjectdansylcadaverine
dc.subjectlc3i protein
dc.subjectlc3ii protein
dc.subjectLiquidambar orientalis extract
dc.subjectmammalian target of rapamycin
dc.subjectphosphatidylinositol 3 kinase
dc.subjectplant extract
dc.subjectprotein
dc.subjectprotein kinase B
dc.subjectunclassified drug
dc.subjectvolatile agent
dc.subjectMTOR protein, human
dc.subjectphosphatidylinositol 3 kinase
dc.subjectplant extract
dc.subjectprotein kinase B
dc.subjecttarget of rapamycin kinase
dc.subjectcancer
dc.subjectcell
dc.subjectendemic species
dc.subjecttoxicity
dc.subjectAkt signaling
dc.subjectapoptosis
dc.subjectArticle
dc.subjectautophagic cell death
dc.subjectcell viability
dc.subjectcontrolled study
dc.subjectcytotoxicity
dc.subjectDU145 cell line
dc.subjectembryo
dc.subjectenzyme linked immunosorbent assay
dc.subjecthuman
dc.subjecthuman cell
dc.subjectIC50
dc.subjectLiquidambar
dc.subjectLiquidambar orientalis
dc.subjectmale
dc.subjectmass fragmentography
dc.subjectPC-3 [Human prostate carcinoma] cell line
dc.subjectprostate cancer
dc.subjecttumor necrosis
dc.subjectWestern blotting
dc.subjectXTT assay
dc.subjectautophagy
dc.subjectmetabolism
dc.subjectprostate tumor
dc.subjectsignal transduction
dc.titleLiquidambar orientalis Mill. gum extract induces autophagy via PI3K/Akt/mTOR signaling pathway in prostate cancer cells
dc.typeArticle

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