Significant Changes in Trans-Epithelial Barrier Proteins of Adenoid Tissue with Atopic Status in Children

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dc.contributor.authorFatma Özge YILMAZ
dc.contributor.authorYurda ŞİMŞEK
dc.contributor.authorSevinç İNAN
dc.contributor.authorÖzlem BUGA
dc.contributor.authorGörkem ESKİİZMİR
dc.contributor.authorErcan PINAR
dc.contributor.authorEsra TOPRAK KANIK
dc.contributor.authorHasan Yüksel GÜNEY
dc.date.accessioned2024-07-24T09:12:26Z
dc.date.available2024-07-24T09:12:26Z
dc.date.issued2020
dc.description.abstractOBJECTIVES: Adenoid tissue is important in local immune response and epithelial barrier dysfunction of this tissue may contribute to allergies. The aim of this study was to evaluate the relationship between the status of cross-epithelial barrier elements in adenoid tissue lymphoepithelium and inhalant allergen sensitization.MATERIALS AND METHODS: Children aged 5-15 years, who underwent adenotonsillectomy, participated in this study. All subjects underwent skin prick testing with environmental inhalant allergens. Occludin, ZO1, e-cadherin, β-catenin, desmoglein, desmoplakin, and connexon-43 were stained immunohistochemically in the adenoid tissues obtained and scored by H-score.RESULTS: We enrolled 76 children, 14 among whom were sensitized to environmental allergens. Among the zonula occludens proteins, median H-scores for occludin, claudin, and ZO-1 were significantly lower in the atopic compared to the nonatopic group respectively (p<0.001). Similarly, median H-scores for e-cadherin and β catenin proteins of the zonula adherens were significantly lower in the atopic group (p<0.001). Both desmoglein and desmoplakin H-scores were significantly lower in the atopic group [60 (50-100) vs 280 (260-300), p<0.001 and 105 (87.5-120) vs 280 (67.25-300), p<0.001 respectively]. Moreover, connexin-43 protein of the gap junction was significantly lower in the atopic group (p<0.001).CONCLUSION: Adenoid tissue, which is the initial point of contact of inhalant allergens demonstrates epithelial barrier junctional protein, changes in children with inhalant allergen sensitization without clinical allergic disease symptoms. Therefore, it may be concluded that epithelial barrier function plays an important role in the development of allergen sensitization versus tolerance.
dc.identifier.DOI-ID10.5152/TurkThoracJ.2019.18207
dc.identifier.urihttp://akademikarsiv.cbu.edu.tr:4000/handle/123456789/24503
dc.language.isoeng
dc.titleSignificant Changes in Trans-Epithelial Barrier Proteins of Adenoid Tissue with Atopic Status in Children
dc.typeAraştırma Makalesi

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