Direct Write Assembly of Graphene/Poly(ε-Caprolactone) Composite Scaffolds and Evaluation of Their Biological Performance Using Mouse Bone Marrow Mesenchymal Stem Cells

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dc.contributor.authorDeliormanlı A.M.
dc.date.accessioned2024-07-22T08:08:29Z
dc.date.available2024-07-22T08:08:29Z
dc.date.issued2019
dc.description.abstractScaffold and mesenchymal stem cell–based cartilage tissue engineering offers a favorable way for the repair and regeneration of injured cartilage. In this study, poly (ε-caprolactone) PCL scaffolds with grid-like structure having periodic lattice was manufactured by robocasting method in the presence of graphene nanoplatelets for cartilage tissue engineering applications. For this purpose, a PCL solution (20 wt%) containing pristine graphene nanopowders in the form of platelets was prepared as printing ink and it was dispensed through a nozzle at room temperature to an ethanol bath at 4 °C. The construction of porous scaffolds was made by a layer-by-layer assembly. Results revealed that graphene additions were not detrimental to deposition process and the structure of the resultant scaffolds. In vitro cell tests indicated that the prepared grid-like graphene/PCL composite scaffolds have good cytocompatibility and non-toxicity for mouse bone marrow mesenchymal stem cells. The stem cells attached and proliferated well on the scaffolds and they also demonstrated a chondrogenic differentiation in the absence of transforming growth factors. © 2019, Springer Science+Business Media, LLC, part of Springer Nature.
dc.identifier.DOI-ID10.1007/s12010-019-02976-5
dc.identifier.issn02732289
dc.identifier.urihttp://akademikarsiv.cbu.edu.tr:4000/handle/123456789/14409
dc.language.isoEnglish
dc.publisherHumana Press Inc.
dc.subjectAnimals
dc.subjectGraphite
dc.subjectMesenchymal Stem Cells
dc.subjectMice
dc.subjectPolyesters
dc.subjectTemperature
dc.subjectTissue Engineering
dc.subjectTissue Scaffolds
dc.subjectBone
dc.subjectCartilage
dc.subjectCell culture
dc.subjectCell engineering
dc.subjectGraphene
dc.subjectScaffolds
dc.subjectStem cells
dc.subjectTissue
dc.subjectTissue regeneration
dc.subjectalcohol
dc.subjectglycosaminoglycan polysulfate
dc.subjectgraphene
dc.subjectink
dc.subjectpolycaprolactone
dc.subjectgraphite
dc.subjectpolycaprolactone
dc.subjectpolyester
dc.subjectCaprolactone
dc.subjectCartilage tissue engineering
dc.subjectChondrogenic differentiation
dc.subjectLayer-by-layer assemblies
dc.subjectMesenchymal stem cell
dc.subjectPoly (epsiloncaprolactone)
dc.subjectRobocasting
dc.subjectTransforming growth factors
dc.subjectanimal cell
dc.subjectArticle
dc.subjectbone marrow derived mesenchymal stem cell
dc.subjectcartilage
dc.subjectcell adhesion
dc.subjectcell differentiation
dc.subjectcell proliferation
dc.subjectcell structure
dc.subjectcell viability
dc.subjectchondrogenesis
dc.subjectcontrolled study
dc.subjectcytotoxicity
dc.subjectdegradation
dc.subjectdigital imaging
dc.subjectin vitro study
dc.subjectmouse
dc.subjectnonhuman
dc.subjectroom temperature
dc.subjectscanning electron microscopy
dc.subjecttemperature
dc.subjecttissue engineering
dc.subjectanimal
dc.subjectchemistry
dc.subjectcytology
dc.subjectmesenchymal stem cell
dc.subjectprocedures
dc.subjecttissue scaffold
dc.subjectScaffolds (biology)
dc.titleDirect Write Assembly of Graphene/Poly(ε-Caprolactone) Composite Scaffolds and Evaluation of Their Biological Performance Using Mouse Bone Marrow Mesenchymal Stem Cells
dc.typeArticle

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