COMET, TUNEL, and TEM analysis of an infertile male with short tail sperm

AYŞEN DURMAZ GÜVEN; SERAP CİLAKER MIÇILI; HAFİZE SEDA VATANSEVER; Cumhur GÜNDÜZ; Hüsnü Alper BAĞRIYANIK; Nurten DİKMEN; EGE NAZAN TAVMERGEN GÖKER; Erol TAVMERGEN

COMET, TUNEL, and TEM analysis of an infertile male with short tail sperm

Date

2015

Authors

AYŞEN DURMAZ GÜVEN

SERAP CİLAKER MIÇILI

HAFİZE SEDA VATANSEVER

Cumhur GÜNDÜZ

Hüsnü Alper BAĞRIYANIK

Nurten DİKMEN

EGE NAZAN TAVMERGEN GÖKER

Erol TAVMERGEN

Abstract

Male infertility is correlated with sperm morphology and sperm DNA damage, which are completely different from that of fertile individuals. Anaccurate sperm DNA damage analysis and ultrastructural examination of the ejaculate provide important support in the clinical evaluation. It issupposed that in the near future, the fertilization rate, pregnancy rate, and miscarriages could be predicted using the combination of these typesof tests in assisted reproductive technologies (ARTs). For this purpose, we report a very rare case of an infertile man having short tail sperm. Theinfertile man and his wife underwent in vitro fertilization (IVF) with intracytoplasmic sperm injection (ICSI). During this process, we examinedthe ultrastructure of the ejaculated sperm with transmission electron microscopy (TEM) and calculated the sperm DNA damage with terminaldeoxynucleotidyl transferase dUTP nick end labeling (TUNEL) and COMET assays. Then, we evaluated the association between sperm DNAdamage and embryo quality.