Browsing by Subject "epithelial mesenchymal transition"
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Item Airway epithelial barrier dysfunction in the pathogenesis and prognosis of respiratory tract diseases in childhood and adulthood(Taylor and Francis Inc., 2017) Yuksel H.; Turkeli A.The lungs are in direct contact with the environment through the tubular structure that constitutes the airway. Starting from the nasal orifice, the airway is exposed to foreign particles including infectious agents, allergens, and other substances that can damage the airways. Therefore, the airway must have a functional epithelial barrier both in the upper and lower airways to protect against these threats. As with the skin, it is likely that the pathogenesis of respiratory diseases is a consequence of epithelial barrier defects in these airways. The characteristics of this system, starting from the beginning of life and extending into maturing and aging, determine the prognosis of respiratory diseases. In this article, we discuss the pathogenesis, clinical phenotype, and prognosis of respiratory diseases from newborns to adulthood in the context of epithelial barrier function and dysfunction. © 2017 Taylor & Francis.Item The Effect of Resveratrol and Quercetin on Epithelial-Mesenchymal Transition in Pancreatic Cancer Stem Cell(Routledge, 2020) Hoca M.; Becer E.; Kabadayı H.; Yücecan S.; Vatansever H.S.Resveratrol and quercetin are phytochemicals that are found in a variety of plants. The aim of this study was to investigate the effect of resveratrol and quercetin on epithelial-mesenchymal transition (EMT) of CD133+ and CD133− pancreatic cancer cells. Cancer stem cells (CD133+ cells) were obtained from the PANC-1 cells by the MiniMACS system. CD133+ and CD133− PANC-1 cells were treated with different concentrations (5, 10, 25, 50, and 100 µM) of resveratrol and quercetin. Cell growth and cytotoxicity were evaluated by MTT assay. Anticancer and anti-metastatic properties of resveratrol and quercetin were determined by immunocytochemistry using antibodies (ACTA-2, IL-1β, N-cadherin, TNF-α, and vimentin). The immunostaining intensity of CD133+ cells was stronger than CD133− cells. ACTA-2, IL-1β, and N-cadherin immunoreactivities were significantly decreased, whereas TNF-α and vimentin immunoreactivities significantly increased in quercetin-treated CD133+ cells. Moreover, N-cadherin and TNF-α immunoreactivities significantly decreased in resveratrol-treated CD133+ cells. The reduction in N-cadherin and ACTA-2 immunoreactivities was higher than the increase in vimentin immunoreactivity, quercetin could prevent EMT to a greater extent than resveratrol in pancreatic cancer stem cells because of the reduced expression of N-cadherin. Quercetin could be more effective in inhibiting metastasis compared to resveratrol. © 2020, © 2019 Taylor & Francis Group, LLC.Item E-Cadherin: An Important Functional Molecule at Respiratory Barrier Between Defence and Dysfunction(Frontiers Media S.A., 2021) Yuksel H.; Ocalan M.; Yilmaz O.While breathing, many microorganisms, harmful environmental particles, allergens, and environmental pollutants enter the human airways. The human respiratory tract is lined with epithelial cells that act as a functional barrier to these harmful factors and provide homeostasis between external and internal environment. Intercellular epithelial junctional proteins play a role in the formation of the barrier. E-cadherin is a calcium-dependent adhesion molecule and one of the most important molecules involved in intercellular epithelial barier formation. E-cadherin is not only physical barrier element but also regulates cell proliferation, differentiation and the immune response to environmental noxious agents through various transcription factors. In this study, we aimed to review the role of E-cadherin in the formation of airway epithelial barier, its status as a result of exposure to various environmental triggers, and respiratory diseases associated with its dysfunction. Moreover, the situations in which its abnormal activation can be noxious would be discussed. © Copyright © 2021 Yuksel, Ocalan and Yilmaz.Item Anti-Tumorigenic Effect of Resveratrol in HepG2 cells Controlled with Cytochrome-c Dependent Cell Death(Mattioli 1885, 2022) Kıyak N.; Becer E.; Vatansever H.S.; Kükner A.Resveratrol is a phytochemical that is regarded as a potential anticancer agent in liver cancer prevention. It had also been shown that resveratrol has role in the prevention of cancer and anti-cancer properties. In this study, we aimed to investigate the effects of resveratrol on cell viability, apoptosis, cellular proliferation, JAK/STAT pathway and epithelial-mesenchymal transition (EMT) in human hepatocellular carcinoma cell (HepG2) line. Cell growth and cytotoxicity were evaluated with MTT assay with different concentrations (5, 10, 25, 50, 100 μM) of resveratrol in HepG2 cells. The distribution of FasL, cyt-c, caspase-3, Ki-67, ACTA2, CD133, JAK2, N-cadherin, vimentin and STAT3 in HepG2 cells were analyzed using indirect immunoperoxidase technique. The effective dose and incubation time for inhibition of cell growth in HepG2 cells was determined as 100 μM for 48 hours. Decreased Ki-67 immunoreactivity following resveratrol application was significant in HepG2 cells. Increased cytc-c, STAT3, vimentin, N-cadherin and CD133 immunoreactivities were significant between resveratrol applicated HepG2 cells and control group. According to our results, resveratrol induced mitochondrial-dependent cell death and suppressed proliferation in HepG2 cells. On the other hand, our results showed that resveratrol stimulated cellular self-protection responses through activation of EMT and STAT3 protein expression in HepG2 cells. © 2022 Mattioli 1885. All rights reserved.Item Destiny of airway disease: interplay between epithelial barrier and the innate immune system(Taylor and Francis Ltd., 2022) Yüksel H.; Tunca S.When the organism encounters a foreign substance, it responds with mutual and regular interactions at different stages of the immune system. In airway diseases, the first encounter is at the epithelial level, where innate immune cells and their responses form the first leg of the protective mechanism. The most important barrier for environmental damage is the epithelial barrier. However, the epithelial barrier is not just a mechanical barrier. The formation of the microbiome on the epithelium and the tolerance or intolerance to environmental factors are vital. This vital balance is maintained between the epithelial surface and the subepithelial innate immune system. This is achieved by the epithelial line, which is a mechanical and functional barrier between them. In this respect, epithelial barrier function preservation has an important role in the development and prognosis of airway disease. © 2021 Taylor & Francis Group, LLC.