Browsing by Subject "tissue degeneration"
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Item Chemoprotective effect of ascorbic acid, α-tocopherol, and selenium on cyclophosphamide-induced toxicity in the rat ovarium(2013) Gürgen S.G.; Erdoĝan D.; Elmas Ç.; Kaplanoĝlu G.T.; Özer Ç.Objective: The aim of the study was to evaluate the protective efficacy of ascorbic acid, α-tocopherol, and selenium by measuring the glutathione (GSH) levels and proliferating cell nuclear antigen (PCNA) and growth differentiation factor-9 (GDF-9) expression in the ovarian tissues of rats treated with cyclophosphamide (CP) therapy. Methods: Female Wistar rats were divided into 5 groups of 6 rats each: (I) control, (II) only CP, (III) CP + ascorbic acid, (IV) CP + α-tocopherol, and (V) CP + selenium. Immunohistochemical stainings and GSH protocol were then applied. Results: Following CP administration, the rats exhibited significantly lower GDF-9 expression in oocytes and PCNA expression in granulosa cells of follicles in all stages of development (P < 0.05). In CP + antioxidant groups (Groups III, IV, V), GDF-9 immunoreaction in oocytes and PCNA immunoreaction in granulosa cells of the developing follicles were found to show an increase towards the levels observed in the control group (P < 0.05). Conclusions: CP was found to cause remarkable degenerative effects in normal ovarian tissue, and we believe that this damage can be reduced and ovarian tissue can be spared from the toxic effects of CP by using antioxidants such as ascorbic acid, α-tocopherol, and selenium. © 2013 Elsevier Inc.Item The protective effect of granulocyte colony-stimulating factor on endometrium and ovary in a rat model of diabetes mellitus(S. Karger AG, 2014) Pala H.G.; Pala E.E.; Artunc Ulkumen B.; Aktug H.; Yavasoglu A.; Korkmaz H.A.; Erbas O.Aims: To evaluate the effect of granulocyte colony-stimulating factor (G-CSF) on the endometrium and ovaries in an experimental diabetes mellitus (DM) rat model. Methods: A total of 18 female Sprague-Dawley albino mature rats (8 weeks, 200-220 g) were used in this study. Diabetes was induced by intraperitoneal (i.p.) injection of streptozocin randomly in 12 rats. No drug was administered to the remainder of the rats (control group, group 1, n = 6). The other 12 rats were randomly divided into 2 groups; 1 ml/kg i.p. saline was given as vehicle to group 2 (diabetic nontreated control group, n = 6) and 100 μg/kg/day of i.p. G-CSF was given to group 3 (G-CSF-treated group, n = 6) for 4 weeks. After 4 weeks, blood samples were collected and hysterectomy with bilateral oophorectomy was performed for histopathological examination. Results: The mean endometrial gland degeneration and stromal fibrosis scores were significantly higher in group 2 compared with groups 1 and 3. Ovarian follicle degeneration, stromal degeneration and stromal fibrosis scores were significantly higher in group 2 compared with groups 1 and 3. Plasma TGF-β and malondialdehyde levels were significantly lower in groups 1 and 3 compared with group 2. Antimüllerian hormone levels were significantly lower in group 2 compared with groups 1 and 3. Conclusion: Glucose toxicity occurred severely in the ovaries and endometrium of the DM rats. After G-CSF treatment, ovarian and endometrial injury and fibrosis scores decreased significantly. The effects of G-CSF in rat models give hope to improved treatment of human DM complications such as premature ovarian failure and endometrial dysfunction. © 2014 S. Karger AG, Basel.Item Therapeutic effect of sunitinib on diabetes mellitus related ovarian injury: An experimental rat model study(Informa Healthcare, 2015) Erbas O.; Pala H.G.; Pala E.E.; Artunc Ulkumen B.; Akman L.; Akman T.; Oltulu F.; Aktug H.; Yavasoglu A.The aim of our study is to investigate the effect of sunitinib on diabetes mellitus related-ovarian injury and fibrosis in rat models. An experimental diabetes mellitus model was created in 16 rats, and eight rats with normal blood glucose levels were included in control group (Group-1). The diabetic rats were divided into two groups:diabetic control group (water given)-Group-2 and sunitinib treatment group-Group-3. After four weeks, bilateral oophorectomy was performed and ovaries were examined histologically. The groups were compared by Student's t-test, analysis of variance (ANOVA) and Mann-Whitney's U-test. There was a significant increase in no-medication (water given) diabetic rat's ovary (Group-2) in terms of follicular degeneration, stromal degeneration, stromal fibrosis and NF-kappaB immune-expression compared with control group normal rats' ovary (Group-1) (p < 0.0001). Stromal degeneration (p = 0.04), stromal fibrosis (p = 0.01), follicular degeneration (p = 0.02), NF-kappaB immune-expression (p = 0.001) significantly decreased in sunitinib-treated diabetic rat's ovary (Group-3) when compared with no-medication (water given) diabetic rat's ovary (Group-2) (p < 0.05). When we used sunitinib in the treatment of diabetic rats, ovarian injury, fibrosis and NF-kappaB immunoexpression decreased significantly. The effects of sunitinib in rat models give hope to the improved treatment of premature ovarian failure due to diabetes mellitus in humans. © 2015 Informa UK Ltd. All rights reserved: reproduction in whole or part not permitted.Item The effect of Paracetamol exposure on hepatic and renal tissues during statin usage(De Gruyter, 2019) Aksun S.; Gökcimen A.; Kahyaoglu F.; Demirci B.Objective: Statins and Paracetamol have widespread use in clinic and both drugs possess similar side effects; therefore, we investigated if drug-interaction occurs when the combination of these two drugs is used during therapy. Materials and methods: A total of 32 (12-15 months old) grown-up male rats were divided into four groups: Control group, RSV group (10 mg/kg Rosuvastatin/daily), APAP group (50 mg/kg Paracetamol/5 days/weekly), RSV + APAP (10 mg/kg Rosuvastatin/daily + 50 mg/kg Paracetamol/5 days/weekly). At the end of 8 weeks of chronic treatment, the blood and tissue samples were taken under the Ketamine and Xylasine anesthesia (50 mg/kg and 5 mg/kg, respectively). Results: In the liver, sinusoidal dilatations, pyknotic nuclei and hemorrhagic foci are more frequently seen in the group receiving combination therapy; although serum liver functions among groups were not significantly different. Kidney histopathologic alterations in APAP and RSV + APAP groups were found more distinct than in RSV alone group. Inducible nitric oxide synthase activity was highly increased with combination therapy in liver and kidney tissues. Conclusion: RSV-Paracetamol interaction may occur as an important drug interaction histopathologically even before it is manifested biochemically in the clinic. © 2019 De Gruyter. All rights reserved.